| Product Name: | Prealbumin Elisa Kits |
| Product Type : | ELISA Kit |
| Code : | CSB-EL007403HU |
| Size : | 96T,5×96T,10×96T |
| Uniprot NO. : | P25101 |
| Abbreviation : | EDNRA |
| Alias : | ETA, ETAR, ETRA, G protein-coupled receptor|endothelin ET-A receptor|endothelin receptor subtype A|endothelin receptor, ET1-specific type|endothelin-1-specific receptor |
| Species : | Human |
| Sample Types : | serum, plasma, tissue homogenates |
| Detect Range : | 47 pg/ml-3000 pg/ml |
| Sensitivity : | 11.75 pg/ml |
| Assay Time : | 1-5h |
| Sample Volume : | 50-100ul |
| Detection Wavelengt : | 450 nm |
| Availability : | 7-14 working days |
| Research Area : | Cancer |
| Protocol : | Instructions
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version. |
| Target details : | This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. |
| HGNC : | 3179 |
| RGD : | 2535 |
| MGI : | 105923 |
| Precision : | | Intra-assay Precision (Precision within an assay): CV%<8% | | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | | | |
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| Linearity : | | To assess the linearity of the assay, samples were spiked with high concentrations of human EDNRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | | Sample | Serum(n=4) | | | | 1:1 | Average % | 103 | | | | Range % | 96-107 | | | | 1:2 | Average % | 100 | | | | Range % | 91-104 | | | | 1:4 | Average % | 88 | | | | Range % | 81-92 | | | | 1:8 | Average % | 94 | | | | Range % | 84-98 | | |
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| Recovery : | | The recovery of human EDNRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | | | | | Sample Type | Average % Recovery | Range | | | | Serum (n=5) | 91 | 84-96 | | | | EDTA plasma (n=4) | 102 | 93-106 | | | | | | | | | | | | | | | | | | | | |
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| Typical Data : | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | | | | | pg/ml | OD1 | OD2 | Average | Corrected | | | | 3000 | 2.761 | 2.685 | 2.723 | 2.549 | | | | 1500 | 2.318 | 2.452 | 2.385 | 2.211 | | | | 750 | 1.845 | 1.989 | 1.917 | 1.743 | | | | 375 | 1.245 | 1.223 | 1.234 | 1.060 | | | | 187.5 | 0.773 | 0.743 | 0.758 | 0.584 | | | | 94 | 0.570 | 0.556 | 0.563 | 0.389 | | | | 47 | 0.384 | 0.374 | 0.379 | 0.205 | | | | 0 | 0.172 | 0.176 | 0.174 | | | |
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